2. No sharp objects (knives, needles, broken glass, etc.) should be exposed in the laboratory. Scissors should be put back into the box immediately after use. Disposable needles should be put into the lunch box for autoclaving; breaking glassware must Collect all debris immediately;
3. Check whether the gloves are leaking before use
4. When there is skin damage on the hands or other exposed parts of the body, all work should be stopped, and the experimental work can be carried out after the wound has healed.
5. If the skin is cut during the poisoning experiment, the wound should be immediately immersed in the disinfectant and the blood should be squeezed out as much as possible
6. When encountering other accidents in the experiment (such as fire, etc.), the source of infection should be eliminated immediately while the accident is eliminated, so as to prevent being infected or infecting other people;
7. After the experiment is completed, the work surface must be disinfected and the UV light must be turned on for 30 minutes
Precautions
1Personal protective equipment should comply with relevant national standards;
2 Based on the hazard assessment, select appropriate personal protective equipment according to different levels of protection requirements
3 There should be clear written regulations, procedures and instructions for the selection, use and maintenance of personal protective equipment;
4 Check carefully before use, and do not use protective equipment with unclear marks, damage or leakage.
Eye protection equipment
1 Safety glasses and goggles
When working in all biosafety laboratories prone to potential eye damage, eye protection measures must be taken.
Safety glasses with side shields can achieve the purpose of protection. When conducting experiments where chemical and biological pollutants may be spilled, goggles should be worn. Wear a face shield when carrying out potentially explosive and using or mixing strong corrosive and strong acid solutions.
2 Eyewash device
If corrosive liquids or biohazardous liquids are splashed into the eyes of workers, they should (or with the help of colleagues) rinse the eye surface with a large amount of slow-flowing water at the nearest eyewash station (eyewash device) for at least 15 to 30 minutes. The accident report form must be filled out immediately after the incident and reported to the supervisor immediately.
Fixed eyewash device
Claim:
1 The eyewash device should be installed in an obvious and accessible place indoors, and the eyewash water pipe should be kept unobstructed, which is convenient for the staff to use in emergency.
2 The staff should master its operation methods.
Shower device and emergency disinfection spray device
1. Provisions
BSL-2 laboratory should have emergency sprinkler device when necessary
BSL-3 laboratories should be equipped with shower devices (cleaning areas), and emergency disinfection spray devices should be installed in semi-contaminated areas if necessary
2. Requirements:
To keep the pipeline unobstructed, the staff must be informed of the emergency disinfection spray
Place the device and train its operation method
In use, a lot of cold water can be used to rinse the contaminated part, and the rinse time needs at least 20 minutes.
If the pollution is caused by chemical spills, rinse with a lot of rapid water. After using the emergency shower, you must fill in the accident report and report to the supervisor immediately
Head and face protective equipment
Mask
Masks can only protect part of the face from biohazardous substances such as blood, body fluids, secretions and feces and other splashes
Only applies to---
Used in BSL-1 or BSL-2 laboratory
1. Protect the entire face:
Must use a standard protective mask to cover the entire face, or use a mask and goggles (or mask and safety glasses)
2. Wear safety glasses or goggles to protect part of the face, but you must wear a mask to protect part of the face or wear a standard protective face shield.
note:
1. Safety goggles or goggles or masks are often worn at the same time when using protective masks.
2. After the experiment, you must first take off the gloves, and then remove the protective mask by hand.
Protective cap
Wearing simple protective caps in biosafety laboratories can protect workers from contamination caused by splashes of chemical and biological hazardous substances on the head (hair).
Simple disposable protective cap
Simple disposable protection made of non-woven fabric
Requirements: The staff should wear protective caps during the experimental operation
Respiratory protective equipment
1. When laboratory operations cannot safely and effectively limit the aerosol within a certain range, respiratory protective equipment is required.
2. The use and maintenance instructions are included in the safety operation procedure manual of the corresponding activities and used in accordance with the instructions and training requirements.
3. Effective equipment for respiratory protection
----Gas mask (mask, personal respirator and positive pressure protective clothing)
use:
1. When carrying out highly dangerous operations (cleaning up spilled infectious substances and aerosols, gas masks can be used for protection.
2. Choose a gas mask according to the type of danger.
Features:
1. There is a replaceable filter in the gas mask, which can protect the wearer from gas, steam, particles and microorganisms and aerosol damage.
2. The filter must match the correct type of gas mask.
3. In order to achieve the ideal protective effect, each gas mask should match the operator's face.
4. Workplace monitoring, medical evaluation and supervision of respirator users should be arranged to ensure that they always use such equipment correctly
5. Individual adaptability tests should be performed on the respirator.
Wear disposable anti-virus equipment correctly
Use points:
(1) Choose a suitable and qualified gas mask when wearing
(2) Cover the nose, mouth and jaw
(3) Fix on the head with a rubber band (elastic band)
(4) Adjust the position of the face and check it
(5) The gas mask should be collapsed when inhaling
(6) There should be no leakage around the mask when exhaling
Remove the disposable gas mask
When removing the mask:
First lift the rubber band (elastic band) under the mask over the head
Then lift the rubber band (elastic band) above the mask to get the mask off the face
It is worth noting that: Disposable gas masks should be disinfected and then discarded after use
Hand protection equipment
Hand protection equipment-gloves
1. Gloves should be used when working in the laboratory.
2. In contact with infectious substances (blood, body fluids, secretions, exudates, as well as contact with mucous membranes and incomplete skin), suitable gloves must be used to protect workers from accidents caused by pollutants splashing or biological contamination. Damage caused
3. If the gloves are contaminated, they should be taken off as early as possible and discarded after disinfection
4. Gloves should meet the requirements of comfort, flexibility, firmness, wear resistance, puncture resistance and tear resistance according to the nature of the operation they are engaged in, and provide adequate protection for the hazards involved
5. Training for laboratory staff to choose gloves, wear and remove them before and after use.
6. Disposable gloves must not be reused
7. Disposable gloves must be disinfected first and then discarded.
Several key points of the standardized use of gloves:
1. The choice of gloves: biosafety laboratories generally use latex rubber or polynitrile or polyvinyl chloride gloves for protection against strong acids, strong alkalis, organic solvents and biohazardous substances
2. Glove inspection: Check whether the gloves are discolored, perforated (leakage) or cracks before using them. You can check the quality of the gloves by immersing the polyester gloves in water to observe whether there are bubbles
3. The use of gloves:
a) Generally, just wear a pair of gloves (BSL-1 laboratory)
b) Two pairs of gloves should be worn when handling infectious substances in a biological safety cabinet (BSL-2 and BSL-3 laboratories)
c) During the operation, the outer gloves are contaminated, immediately spray the gloves with disinfectant, take them off and discard them in the autoclave bag in the biological safety cabinet, and immediately put on new gloves to continue the experiment.
d) After wearing gloves, you can completely cover your hands and wrists. If necessary, you can cover the sleeves of the lab coat or coat
4. Cleaning and replacement of gloves
a) Use disposable gloves and do not reuse them. Autoclave immediately after use and then discard.
b) Do not leave the laboratory area wearing gloves.
c) Replace gloves if they are torn, damaged or suspected of internal contamination
d) Wash hands immediately after taking off gloves.
e) The staff should remove the outer gloves and throw them into the autoclave bag in the biological safety cabinet before leaving the biological safety cabinet after completing the infectious substance experiment. Then spray the inner gloves with disinfectant to avoid contamination of door knobs, light switches, telephones, etc. (BSL-2 and BSL-3 laboratories)
5Avoid “touch pollution” with gloves
a) Wear gloved hands to avoid touching the nose, face and avoid touching or adjusting other protective equipment (such as glasses, etc.)
b) Avoid touching unnecessary surfaces such as light switches, doors or knobs, etc.
c) If gloves are torn, they should be taken off, and hands should be washed before putting on new gloves
Be careful not to touch the work surface and other objects
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